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Accurate identification of allergen sensitization plays a crucial role in the management of asthma and allergic rhinitis (AR), with diagnostic approaches such as skin prick test (SPT) and ImmunoCAP widely used in clinical practice.
ALEX-2 shows moderate concordance with ImmunoCAP and skin prick testing, with the strongest agreement observed for dust mite allergen sensitization.
Accurate identification of allergen sensitization plays a crucial role in the management of asthma and allergic rhinitis (AR), with diagnostic approaches such as skin prick test (SPT) and ImmunoCAP widely used in clinical practice. However, these tests typically evaluate a limited number of allergens per panel, whereas the recently developed allergy explorer 2 (ALEX-2) multiplex platform enables simultaneous assessment of multiple allergen components, yet comparative data evaluating its performance in Latin American populations remain limited. Hence, this study assessed the concordance between ALEX-2, SPT, and ImmunoCAP in identifying respiratory allergen sensitization among patients with asthma or AR.
Researchers conducted a prospective cross-sectional study involving 57 patients diagnosed with asthma or AR who were consecutively recruited from a private allergy clinic in Lima, Peru. Each participant underwent evaluation using 3 diagnostic approaches:
The study focused on determining the level of agreement between these diagnostic tools in detecting sensitization to respiratory allergens. Statistical concordance between the tests was analyzed using Cohen’s kappa (κ) coefficient.
The strongest overall agreement was observed between ALEX-2 and SPT (κ = 0.5649), followed by ALEX-2 and ImmunoCAP (κ = 0.4911), and ImmunoCAP and SPT (κ = 0.4688).
The ALEX-2 multiplex platform demonstrated consistent moderate concordance with established allergy tests like SPT and ImmunoCAP, especially for dust mite sensitization. These findings support its role as a complementary diagnostic tool in respiratory allergy evaluation, although further large-scale validation studies are needed.
Immunity, Inflammation and Disease
Concordance Analysis of ALEX-2, ImmunoCAP, and Prick Test for Respiratory Allergies in Lima, Peru
César A Galván et al.
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