Inflammation in low back pain may be detected from the peripheral blood: suggestions for biomarker

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Inflammation in low back pain may be detected from the peripheral blood: suggestions for biomarker
Key Take-Away: 

Low back pain is the most frequently observed condition throughout the world, but the basis for low back pain has been poorly understood. The researchers of this present study furnish many novel evidences regarding the mechanisms of neuro-immune inflammation that occurs with the low back pain. They have tried to put light on how biochemical and cellular alterations are beneficial as biomarkers for knowing or studying a disease.

Biomarker for prediction of development of low back pain, and disease progression in chronic conditions are virtually non-existent.

ABSTRACT: 
Background: 

Biomarker for prediction of development of low back pain, and disease progression in chronic conditions are virtually non-existent.

Methods: 

In the present study, we examined evidence of inflammation in the peripheral blood and demonstrated significant changes in neuroinflammation markers in subjects with chronic low back pain in comparison to control subjects.

The present study was performed using peripheral blood from subjects with chronic low back pain and age-matched control subjects. Western blotting, real-time RT-PCR, cell culture and in vitro assays were incorporated to perform the current study.

Results: 

We obtained evidence that the balance between proinflammatory and anti-inflammatory cytokines is misaligned, with decrease in interleukin-10 (IL-10) expression and increase in interleukin-6 (IL-6) expression.

Furthermore, we demonstrated increase in CD16 monocyte expression. Cells were cultured under differential conditions to generate M1/M2 macrophages. In the macrophages, opioid secretory capacity was shown to be diminished. Finally, Dragon (repulsive guidance molecule b, RGMb) expression was shown diminished in M1 macrophages, which serves as a key transcriptional inhibitor of IL-6 expression.

Conclusion: 

These biochemical and cellular alterations in chronic low back pain can serve as potential biomarkers for assessing disease initiation, intensity and progression.

Biosci Rep. 2016 Aug 5;36(4):e00361
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